Hotspot

trnK-UUU-rps16

LSC · 4302-5333 · 1032 bp

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Pi 0.0275
MarkerSeek score 69.80
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
1032
variable_sites
73
pi
0.0275
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT GGGCAGGGGTTTTTAAGGAA 50.0 50.0 58.2 58.0 1104-1195 bp 1.0 87.6
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT GGGCAGGGGTTTTTAAGGAA 50.0 50.0 58.2 58.0 1103-1194 bp 1.0 87.6
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT AAGGGCAGGGGTTTTTAAGG 50.0 50.0 58.2 58.0 1106-1197 bp 1.0 87.5
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT AAGGGCAGGGGTTTTTAAGG 50.0 50.0 58.2 58.0 1105-1196 bp 1.0 87.5
trnK-UUU-rps16_p5 CCGCACTTAAAAGCCGAGTA GGGCAGGGGTTTTTAAGGAA 50.0 50.0 58.3 58.0 1113-1204 bp 1.0 86.6