Hotspot

trnH-GUG-psbA

LSC · 90-307 · 218 bp

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Pi 0.0581
MarkerSeek score 63.40
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
218
variable_sites
28
pi
0.0581
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.90
markerseek_score
63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG AGCTGCTGTTGAAGTTCCAT 55.0 45.0 58.1 57.7 240-273 bp 1.0 88.7
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 254-287 bp 1.0 88.7
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 253-286 bp 1.0 88.7
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC AGCTGCTGTTGAAGTTCCAT 50.0 45.0 57.9 57.7 255-288 bp 1.0 88.7
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG GCTGCTGTTGAAGTTCCATC 55.0 50.0 58.1 57.4 239-272 bp 1.0 86.0