Hotspot

ycf1

SSC · 108051-113261 · 5211 bp

Back to Gerbera

Pi 0.00192

MarkerSeek score 42.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5211
variable_sites: 10
pi: 0.00192
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 42.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAATTCCCATTCCCCGCTTT	TGAGCTAGTAACAGAACACTTCT	45.0	39.1	58.0	57.0	228 bp	1.0	48.3
ycf1_p2	AATTCCCATTCCCCGCTTTT	TGAGCTAGTAACAGAACACTTCT	45.0	39.1	58.0	57.0	227 bp	1.0	48.3
ycf1_p3	ATTCCCATTCCCCGCTTTTT	TGAGCTAGTAACAGAACACTTCT	45.0	39.1	58.0	57.0	226 bp	1.0	48.3
ycf1_p4	AGTAAATTCCCATTCCCCGC	TGAGCTAGTAACAGAACACTTCT	50.0	39.1	57.9	57.0	231 bp	1.0	48.1
ycf1_p5	GTAAATTCCCATTCCCCGCT	TGAGCTAGTAACAGAACACTTCT	50.0	39.1	57.9	57.0	230 bp	1.0	48.1