Hotspot

rpl32-ndhF

SSC · 123528-124579 · 1052 bp

Back to Gerbera

Pi 0.00389

MarkerSeek score 63.20

Primer available yes

Label rpl32-ndhF

Feature Metadata

label_name: rpl32-ndhF
feature_type: igs
region: SSC
length_bp: 1052
variable_sites: 4
pi: 0.00389
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-ndhF_p1	ACGCCGACCAATATCCTTTC	CTTTTTGTTGCCGTCGGAAA	50.0	45.0	58.1	57.8	1217-1241 bp	1.0	79.8
rpl32-ndhF_p2	CAAAGCTTTTAACGCCGACC	CTTTTTGTTGCCGTCGGAAA	50.0	45.0	58.3	57.8	1228-1252 bp	1.0	77.7
rpl32-ndhF_p3	ACGCCGACCAATATCCTTTC	AAGACTTTTTGTTGCCGTCG	50.0	45.0	58.1	57.5	1221-1245 bp	1.0	77.7
rpl32-ndhF_p4	ACGCCGACCAATATCCTTTC	ACGAAGACTTTTTGTTGCCG	50.0	45.0	58.1	57.5	1224-1248 bp	1.0	77.7
rpl32-ndhF_p5	ACGCCGACCAATATCCTTTC	CGAAGACTTTTTGTTGCCGT	50.0	45.0	58.1	57.5	1223-1247 bp	1.0	77.7