Hotspot

rpl22-rps19

LSC · 83185-83247 · 63 bp

Back to Gerbera

Pi 0.0159

MarkerSeek score 61.60

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 63
variable_sites: 1
pi: 0.0159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CGTAGGAACGTCCACGAATT	TCCGAGGACATGCAAAAAGC	50.0	50.0	58.0	59.1	210-212 bp	1.0	71.3
rpl22-rps19_p2	ATCAATTACCCTTCGCGCTT	TCCGAGGACATGCAAAAAGC	45.0	50.0	58.0	59.1	188-190 bp	1.0	71.2
rpl22-rps19_p3	CGTAGGAACGTCCACGAATT	AACAGATCGTATGGTAGGCC	50.0	50.0	58.0	56.8	263-265 bp	1.0	70.6
rpl22-rps19_p4	CAATTACCCTTCGCGCTTTG	TCCGAGGACATGCAAAAAGC	50.0	50.0	58.1	59.1	186-188 bp	1.0	70.6
rpl22-rps19_p5	ATCAATTACCCTTCGCGCTT	AACAGATCGTATGGTAGGCC	45.0	50.0	58.0	56.8	241-243 bp	1.0	70.4