Hotspot

trnE-UUC-rpoB

LSC · 11725-12524 · 800 bp

Back to Gerbera

Pi 0.0025

MarkerSeek score 60.20

Primer available yes

Label trnE-UUC-rpoB

Feature Metadata

label_name: trnE-UUC-rpoB
feature_type: igs
region: LSC
length_bp: 800
variable_sites: 2
pi: 0.0025
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-rpoB_p1	AGCGTCTTAAATGAAATCAACGG	AACCCGGGGATTGTAGACAT	39.1	50.0	58.0	58.4	854 bp	1.0	52.7
trnE-UUC-rpoB_p2	AGCGTCTTAAATGAAATCAACGG	AAACCCGGGGATTGTAGACA	39.1	50.0	58.0	58.6	855 bp	1.0	50.9
trnE-UUC-rpoB_p3	GTCTTAAATGAAATCAACGGGCT	AACCCGGGGATTGTAGACAT	39.1	50.0	57.7	58.4	851 bp	1.0	50.4
trnE-UUC-rpoB_p4	GCGTCTTAAATGAAATCAACGG	AACCCGGGGATTGTAGACAT	40.9	50.0	56.7	58.4	853 bp	1.0	50.4
trnE-UUC-rpoB_p5	GTCTTAAATGAAATCAACGGGCT	AAACCCGGGGATTGTAGACA	39.1	50.0	57.7	58.6	852 bp	1.0	48.6