Hotspot

rps11

Genome · 141073-142023 · 951 bp

Back to Geranium

Pi 0.0535
MarkerSeek score 89.50
Primer available yes
Label rps11

Feature Metadata

label_name
rps11
feature_type
gene
region
Genome
length_bp
951
variable_sites
50
pi
0.0535
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
89.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps11_p1 CAGTTTCGTTTTAACATGAGATGGA AAGCAGGCTTTGTAACTCCC 36.0 50.0 58.1 58.1 480-494 bp 1.0 48.3
rps11_p2 CAGTTTCGTTTTAACATGAGATGGA GCAGGCTTTGTAACTCCCTT 36.0 50.0 58.1 58.1 478-492 bp 1.0 48.3
rps11_p3 TCAGTTTCGTTTTAACATGAGATGG AAGCAGGCTTTGTAACTCCC 36.0 50.0 58.1 58.1 481-495 bp 1.0 48.3
rps11_p4 TCAGTTTCGTTTTAACATGAGATGG GCAGGCTTTGTAACTCCCTT 36.0 50.0 58.1 58.1 479-493 bp 1.0 48.3
rps11_p5 AGTTTCGTTTTAACATGAGATGGA GCAGGCTTTGTAACTCCCTT 33.3 50.0 56.9 58.1 477-491 bp 1.0 48.3
rps11_p1 TTATTGTTGGGAAGGCCAGG CAGTTTCGTTTTAACATGAGATGGA 50.0 36.0 57.8 58.1 1085-1087 bp 1.0 46.2
rps11_p2 TTATTGTTGGGAAGGCCAGG TCAGTTTCGTTTTAACATGAGATGG 50.0 36.0 57.8 58.1 1086-1088 bp 1.0 46.2
rps11_p3 TTATTGTTGGGAAGGCCAGG AGTTTCGTTTTAACATGAGATGGA 50.0 33.3 57.8 56.9 1084-1086 bp 1.0 46.2
rps11_p4 TTATTGTTGGGAAGGCCAGG CAGTTTCGTTTTAACATGAGATGG 50.0 37.5 57.8 56.8 1085-1087 bp 1.0 46.2
rps11_p5 TTATTGTTGGGAAGGCCAGG GTTTCGTTTTAACATGAGATGGA 50.0 34.8 57.8 55.5 1083-1085 bp 1.0 46.2