Hotspot

rpl23

Genome · 64418-65967 · 1550 bp

Back to Geranium

Pi 0.0739

MarkerSeek score 76.90

Primer available yes

Label rpl23

Feature Metadata

label_name: rpl23
feature_type: gene
region: Genome
length_bp: 1550
variable_sites: 113
pi: 0.0739
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23_p1	CCACAACGATGCTGTCCATA	CGACACCAGACAATACCCAG	50.0	55.0	58.0	58.0	743-2276 bp	1.0	88.8
rpl23_p2	CGCCTCTGACCATCAAATGA	CGACACCAGACAATACCCAG	50.0	55.0	58.0	58.0	1333-2866 bp	1.0	87.4
rpl23_p3	CAATTCGCCTCTGACCATCA	CGACACCAGACAATACCCAG	50.0	55.0	58.0	58.0	1338-2871 bp	1.0	87.3
rpl23_p4	TCGCCTCTGACCATCAAATG	CGACACCAGACAATACCCAG	50.0	55.0	58.0	58.0	1334-2867 bp	1.0	87.3
rpl23_p5	CCACAACGATGCTGTCCATA	GGGGATTCATGGCGAAGATT	50.0	50.0	58.0	58.0	2359 bp	0.5	59.7