Hotspot

petA-psbJ

Genome · 128100-129345 · 1246 bp

Back to Geranium

Pi 0.0238

MarkerSeek score 74.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 1246
variable_sites: 29
pi: 0.0238
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGTGCTTCTTGGCATCTGTT	GCTGGTATTCCTGTGATCGG	45.0	55.0	58.0	58.1	1390-1416 bp	1.0	82.0
petA-psbJ_p2	TTGTGCTTCTTGGCATCTGT	GCTGGTATTCCTGTGATCGG	45.0	55.0	58.0	58.1	1391-1417 bp	1.0	82.0
petA-psbJ_p3	TCAAGGCCTTTTGTGCTTCT	GCTGGTATTCCTGTGATCGG	45.0	55.0	57.9	58.1	1401-1427 bp	1.0	81.6
petA-psbJ_p4	CGTGTTCAAGGCCTTTTGTG	GCTGGTATTCCTGTGATCGG	50.0	55.0	58.2	58.1	1406-1432 bp	1.0	80.8
petA-psbJ_p5	TGTGCTTCTTGGCATCTGTT	ATACTCCGGGTTGGGTTCAT	45.0	50.0	58.0	58.4	1343-1369 bp	1.0	79.9