Hotspot

rrn16S-trnV

Genome · 58011-58822 · 812 bp

Back to Geranium

Pi 0.0511

MarkerSeek score 79.10

Primer available yes

Label rrn16S-trnV

Feature Metadata

label_name: rrn16S-trnV
feature_type: igs
region: Genome
length_bp: 812
variable_sites: 38
pi: 0.0511
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16S-trnV_p1	TTTCCAGCTGTTGTTCCCC	GGGGCAGGGAAGGGATATAA	52.6	55.0	57.9	58.2	930-1102 bp	1.0	76.0
rrn16S-trnV_p2	TTTCCAGCTGTTGTTCCCC	GACGAGGTGGAAGTCATCAG	52.6	55.0	57.9	57.7	888-1060 bp	1.0	75.8
rrn16S-trnV_p3	TTTCCAGCTGTTGTTCCCC	GGAAGTCATCAGTTCGAGCC	52.6	55.0	57.9	58.4	880-1052 bp	1.0	75.3
rrn16S-trnV_p4	TTTCCAGCTGTTGTTCCCC	ACGAGGTGGAAGTCATCAGT	52.6	50.0	57.9	58.4	887-1059 bp	1.0	75.2
rrn16S-trnV_p5	TTTCCAGCTGTTGTTCCCC	TGGAAGTCATCAGTTCGAGC	52.6	50.0	57.9	57.6	881-1053 bp	1.0	75.2