Hotspot

trnI-rrn16S

Genome · 53914-56534 · 2621 bp

Back to Geranium

Pi 0.0669

MarkerSeek score 79.30

Primer available yes

Label trnI-rrn16S

Feature Metadata

label_name: trnI-rrn16S
feature_type: igs
region: Genome
length_bp: 2621
variable_sites: 173
pi: 0.0669
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-rrn16S_p1	CTATCCATGTGGCTGAGAGC	GAAGGTGTGGCTGGATTACC	55.0	55.0	57.8	58.2	417-2731 bp	1.0	86.4
trnI-rrn16S_p2	CTATCCATGTGGCTGAGAGC	GGAAGGTGTGGCTGGATTAC	55.0	55.0	57.8	58.2	418-2732 bp	1.0	86.4
trnI-rrn16S_p3	CTATCCATGTGGCTGAGAGC	CAAGGTAGCCGTACTGGAAG	55.0	55.0	57.8	57.7	433-2747 bp	1.0	86.0
trnI-rrn16S_p4	GGCACAACGACGCAATTATC	GAAGGTGTGGCTGGATTACC	50.0	55.0	58.2	58.2	2699-2990 bp	1.0	82.3
trnI-rrn16S_p5	GGCACAACGACGCAATTATC	GGAAGGTGTGGCTGGATTAC	50.0	55.0	58.2	58.2	2700-2991 bp	1.0	82.3