Hotspot

rps11-rpoA

Genome · 16212-16281 · 70 bp

Back to Geranium

Pi 0.0781

MarkerSeek score 74.60

Primer available yes

Label rps11-rpoA

Feature Metadata

label_name: rps11-rpoA
feature_type: igs
region: Genome
length_bp: 70
variable_sites: 5
pi: 0.0781
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpoA_p1	AACCCCTATGCCACATAACG	GACTCTTACTTTTTCTCGAATCA	50.0	34.8	57.7	54.5	140-146 bp	1.0	46.2
rps11-rpoA_p2	AACCCCTATGCCACATAACG	ACTCTTACTTTTTCTCGAATCA	50.0	31.8	57.7	53.2	139-145 bp	1.0	46.2
rps11-rpoA_p3	AACCCCTATGCCACATAACG	GACTCTTACTTTTTCTCGAATCAT	50.0	33.3	57.7	55.0	140-146 bp	1.0	46.2
rps11-rpoA_p4	AACCCCTATGCCACATAACG	ACTCTTACTTTTTCTCGAATCAT	50.0	30.4	57.7	53.8	139-145 bp	1.0	46.2
rps11-rpoA_p5	CGGGATATAACCCCTATGCC	GACTCTTACTTTTTCTCGAATCA	55.0	34.8	57.0	54.5	148-154 bp	1.0	45.8