Hotspot

rpl14-rps8

Genome · 14542-14628 · 87 bp

Back to Geranium

Pi 0.0706

MarkerSeek score 80.40

Primer available yes

Label rpl14-rps8

Feature Metadata

label_name: rpl14-rps8
feature_type: igs
region: Genome
length_bp: 87
variable_sites: 6
pi: 0.0706
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rps8_p1	AATTTCCCTAGCGCCTGAAG	AACCGTCTCTTTGTTAGCCA	50.0	45.0	58.0	57.1	185-187 bp	1.0	75.7
rpl14-rps8_p2	GTGCAATCACCCACGAATTG	AACCGTCTCTTTGTTAGCCA	50.0	45.0	58.0	57.1	228-230 bp	1.0	75.3
rpl14-rps8_p3	TGCAATCACCCACGAATTGA	AACCGTCTCTTTGTTAGCCA	45.0	45.0	58.1	57.1	227-229 bp	1.0	74.6
rpl14-rps8_p4	AATCCAAAAGGAACTCGCGT	AACCGTCTCTTTGTTAGCCA	45.0	45.0	58.1	57.1	253-255 bp	1.0	74.3
rpl14-rps8_p5	ATCCAAAAGGAACTCGCGTT	AACCGTCTCTTTGTTAGCCA	45.0	45.0	58.1	57.1	252-254 bp	1.0	74.3