Hotspot

trnI-rpl16

Genome · 12878-13610 · 733 bp

Back to Geranium

Pi 0.0569

MarkerSeek score 81.00

Primer available yes

Label trnI-rpl16

Feature Metadata

label_name: trnI-rpl16
feature_type: igs
region: Genome
length_bp: 733
variable_sites: 41
pi: 0.0569
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-rpl16_p1	GTTGGGCGCTTTAACCATTC	AATACGACTGCCTTGAGAGG	50.0	50.0	58.0	57.0	844-846 bp	1.0	77.3
trnI-rpl16_p2	GTTGGGCGCTTTAACCATTC	CCGAAAGAAATACGACTGCC	50.0	50.0	58.0	56.9	852-854 bp	1.0	76.3
trnI-rpl16_p3	GTTGGGCGCTTTAACCATTC	AAATACGACTGCCTTGAGAGG	50.0	47.6	58.0	57.7	845-847 bp	1.0	75.0
trnI-rpl16_p4	AGTTGGGCGCTTTAACCATT	AATACGACTGCCTTGAGAGG	45.0	50.0	58.4	57.0	845-847 bp	1.0	74.4
trnI-rpl16_p5	TTGGGCGCTTTAACCATTCA	AATACGACTGCCTTGAGAGG	45.0	50.0	58.4	57.0	843-845 bp	1.0	74.3