Hotspot

ycf80

Genome · 70713-72173 · 1461 bp

Back to Gephyrocapsa

Pi 0.000913

MarkerSeek score 59.30

Primer available yes

Label ycf80

Feature Metadata

label_name: ycf80
feature_type: gene
region: Genome
length_bp: 1461
variable_sites: 2
pi: 0.000913
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf80_p1	ATTCCAAAACAGCCCCTTGG	CCCCTAACCGCGGATTTATC	50.0	55.0	58.6	58.2	1652 bp	1.0	73.3
ycf80_p2	CGATTCCAAAACAGCCCCTT	CCCCTAACCGCGGATTTATC	50.0	55.0	58.7	58.2	1654 bp	1.0	72.6
ycf80_p3	GATTCCAAAACAGCCCCTTG	CCCCTAACCGCGGATTTATC	50.0	55.0	57.3	58.2	1653 bp	1.0	72.5
ycf80_p4	TGTCGATTCCAAAACAGCCC	CCCCTAACCGCGGATTTATC	50.0	55.0	58.8	58.2	1657 bp	1.0	72.5
ycf80_p5	ATTCCAAAACAGCCCCTTGG	CCCTAACCGCGGATTTATCA	50.0	50.0	58.6	57.5	1651 bp	1.0	70.6