Hotspot

ycf39

Genome · 104210-105130 · 921 bp

Back to Gephyrocapsa

Pi 0.00217

MarkerSeek score 60.10

Primer available yes

Label ycf39

Feature Metadata

label_name: ycf39
feature_type: gene
region: Genome
length_bp: 921
variable_sites: 3
pi: 0.00217
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf39_p1	GCGGTGCTCTAACCAATTGA	TGATTTCTGGGGCTGTTTTT	50.0	40.0	58.6	55.7	1156 bp	1.0	57.5
ycf39_p2	GCGGTGCTCTAACCAATTGA	AATGATTTCTGGGGCTGTTT	50.0	40.0	58.6	55.5	1158 bp	1.0	55.6
ycf39_p3	GCGGTGCTCTAACCAATTGA	ATGATTTCTGGGGCTGTTTT	50.0	40.0	58.6	55.5	1157 bp	1.0	55.6
ycf39_p4	GCGGTGCTCTAACCAATTGA	TTCTGGGGCTGTTTTTACAA	50.0	40.0	58.6	55.4	1152 bp	1.0	54.7
ycf39_p5	GCGGTGCTCTAACCAATTGA	TTTCTGGGGCTGTTTTTACA	50.0	40.0	58.6	55.4	1153 bp	1.0	54.7