Hotspot

secY

Genome · 97417-98685 · 1269 bp

Back to Gephyrocapsa

Pi 0.00158

MarkerSeek score 59.80

Primer available yes

Label secY

Feature Metadata

label_name: secY
feature_type: gene
region: Genome
length_bp: 1269
variable_sites: 3
pi: 0.00158
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
secY_p1	TTTTAAATAATGCGCGGGCG	AGTCGCGTATTAAACCATTAACT	45.0	34.8	58.2	56.4	1470 bp	1.0	41.8
secY_p2	GGCGACAATCGATGGTCTTA	AGTCGCGTATTAAACCATTAACT	50.0	34.8	57.8	56.4	1454 bp	1.0	41.4
secY_p3	TTTTAAATAATGCGCGGGCG	AGTCGCGTATTAAACCATTAAC	45.0	36.4	58.2	55.0	1470 bp	1.0	40.2
secY_p4	TTTTAAATAATGCGCGGGCG	GTCGCGTATTAAACCATTAACT	45.0	36.4	58.2	55.0	1469 bp	1.0	40.2
secY_p5	GGCGACAATCGATGGTCTTA	GTCGCGTATTAAACCATTAACT	50.0	36.4	57.8	55.0	1453 bp	1.0	40.2