Hotspot

secA

Genome · 79226-81871 · 2646 bp

Back to Gephyrocapsa

Pi 0.000756

MarkerSeek score 30.80

Primer available yes

Label secA

Feature Metadata

label_name: secA
feature_type: gene
region: Genome
length_bp: 2646
variable_sites: 3
pi: 0.000756
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 30.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
secA_p1	AGAGGAAACCAATTTGGGCT	CTGCTCCGTCTCCCTATAGA	45.0	55.0	57.3	57.4	2825 bp	1.0	70.0
secA_p2	AAGAGGAAACCAATTTGGGCT	CTGCTCCGTCTCCCTATAGA	42.9	55.0	58.0	57.4	2826 bp	1.0	67.5
secA_p3	AGAGGAAACCAATTTGGGCT	GCTGCTCCGTCTCCCTATA	45.0	57.9	57.3	58.0	2826 bp	1.0	66.3
secA_p4	AGAGGAAACCAATTTGGGCT	TGCTCCGTCTCCCTATAGAA	45.0	50.0	57.3	57.0	2824 bp	1.0	66.3
secA_p5	AGAGGAAACCAATTTGGGCT	GCTGCTCCGTCTCCCTATAG	45.0	60.0	57.3	59.1	2826 bp	1.0	65.7