Hotspot

rpoA

Genome · 99848-100777 · 930 bp

Back to Gephyrocapsa

Pi 0.00358

MarkerSeek score 61.00

Primer available yes

Label rpoA

Feature Metadata

label_name: rpoA
feature_type: gene
region: Genome
length_bp: 930
variable_sites: 5
pi: 0.00358
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA_p1	CTGTCCCTCACAATGGCTG	TGATACCAGTCCCAGGTTGT	57.9	50.0	58.1	58.0	1135 bp	1.0	71.0
rpoA_p2	CTGTCCCTCACAATGGCTG	GCTTCTTTTCGCCTTCCAAC	57.9	50.0	58.1	57.9	1097 bp	1.0	70.9
rpoA_p3	TACACCTGTCCCTCACAATG	TGATACCAGTCCCAGGTTGT	50.0	50.0	56.9	58.0	1140 bp	1.0	70.8
rpoA_p4	TACACCTGTCCCTCACAATG	GCTTCTTTTCGCCTTCCAAC	50.0	50.0	56.9	57.9	1102 bp	1.0	70.7
rpoA_p5	CACCTGTCCCTCACAATGG	TGATACCAGTCCCAGGTTGT	57.9	50.0	57.7	58.0	1138 bp	1.0	70.0