Hotspot

rbcS

Genome · 12973-13392 · 420 bp

Back to Gephyrocapsa

Pi 0.00159

MarkerSeek score 29.70

Primer available yes

Label rbcS

Feature Metadata

label_name: rbcS
feature_type: gene
region: Genome
length_bp: 420
variable_sites: 1
pi: 0.00159
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcS_p1	TTCTAGCACGTAACGAAGGC	GAGCTTGGTACTACAGCACC	50.0	55.0	58.0	58.0	1192 bp	1.0	80.3
rbcS_p2	GTGGTCCTCTTCAAACTGCA	GAGCTTGGTACTACAGCACC	50.0	55.0	58.0	58.0	1120 bp	1.0	80.2
rbcS_p3	TGGATGCGTATGTCAGGTTG	GAGCTTGGTACTACAGCACC	50.0	55.0	58.0	58.0	1526 bp	1.0	80.1
rbcS_p4	GGATGCGTATGTCAGGTTGT	GAGCTTGGTACTACAGCACC	50.0	55.0	58.0	58.0	1525 bp	1.0	80.1
rbcS_p5	GTGGATGCGTATGTCAGGTT	GAGCTTGGTACTACAGCACC	50.0	55.0	58.0	58.0	1527 bp	1.0	80.1