Hotspot

petG-secA

Genome · 79175-79225 · 51 bp

Back to Gephyrocapsa

Pi 0.0654
MarkerSeek score 74.80
Primer available yes
Label petG-secA

Feature Metadata

label_name
petG-secA
feature_type
igs
region
Genome
length_bp
51
variable_sites
5
pi
0.0654
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petG-secA_p1 AGAGGAAACCAATTTGGGCT AATCGTATCCGTCATCAGGT 45.0 45.0 57.3 56.1 141 bp 1.0 62.3
petG-secA_p2 AGAGGAAACCAATTTGGGCT AAATCGTATCCGTCATCAGGT 45.0 42.9 57.3 56.9 142 bp 1.0 60.4
petG-secA_p3 AAGAGGAAACCAATTTGGGCT AATCGTATCCGTCATCAGGT 42.9 45.0 58.0 56.1 142 bp 1.0 59.7
petG-secA_p4 AAGAGGAAACCAATTTGGGCT AAATCGTATCCGTCATCAGGT 42.9 42.9 58.0 56.9 143 bp 1.0 57.8
petG-secA_p5 GAAGAGGAAACCAATTTGGGC AATCGTATCCGTCATCAGGT 47.6 45.0 57.7 56.1 143 bp 1.0 57.3