Hotspot

rbcR-trnE-UUC

Genome · 76671-76852 · 182 bp

Back to Gephyrocapsa

Pi 0.00733

MarkerSeek score 49.60

Primer available yes

Label rbcR-trnE-UUC

Feature Metadata

label_name: rbcR-trnE-UUC
feature_type: igs
region: Genome
length_bp: 182
variable_sites: 2
pi: 0.00733
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcR-trnE-UUC_p1	ACTTCAGCAGCCTCAACAAA	ATGTCCTAGGCCTCTAGACG	45.0	55.0	57.9	57.7	240 bp	1.0	78.0
rbcR-trnE-UUC_p2	ACTTCAGCAGCCTCAACAAA	CCTAGGCCTCTAGACGATGG	45.0	60.0	57.9	58.5	236 bp	1.0	76.5
rbcR-trnE-UUC_p3	ACTTCAGCAGCCTCAACAAA	CTAGGCCTCTAGACGATGGG	45.0	60.0	57.9	58.5	235 bp	1.0	76.5
rbcR-trnE-UUC_p4	ACTTCAGCAGCCTCAACAAA	AAAGGGAGATGTCCTAGGCC	45.0	55.0	57.9	58.5	248 bp	1.0	76.3
rbcR-trnE-UUC_p5	ACTTCAGCAGCCTCAACAAA	TCCTAGGCCTCTAGACGATG	45.0	55.0	57.9	57.4	237 bp	1.0	75.7