Hotspot

trnR-ACG-trnS-UGA

Genome · 65153-65478 · 326 bp

Back to Gephyrocapsa

Pi 0.00409
MarkerSeek score 35.60
Primer available yes
Label trnR-ACG-trnS-UGA

Feature Metadata

label_name
trnR-ACG-trnS-UGA
feature_type
igs
region
Genome
length_bp
326
variable_sites
2
pi
0.00409
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
35.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnS-UGA_p1 AAAGGGCTTGTAGCTCAGTG GGTTCGAATCCCACCTTCTC 50.0 55.0 57.8 58.0 425 bp 1.0 78.6
trnR-ACG-trnS-UGA_p2 AAAGGGCTTGTAGCTCAGTG GTTCGAATCCCACCTTCTCC 50.0 55.0 57.8 58.0 424 bp 1.0 78.6
trnR-ACG-trnS-UGA_p3 ACAAAGGGCTTGTAGCTCAG GTTCGAATCCCACCTTCTCC 50.0 55.0 57.8 58.0 426 bp 1.0 78.6
trnR-ACG-trnS-UGA_p4 CAAAGGGCTTGTAGCTCAGT GGTTCGAATCCCACCTTCTC 50.0 55.0 57.8 58.0 426 bp 1.0 78.6
trnR-ACG-trnS-UGA_p5 CAAAGGGCTTGTAGCTCAGT GTTCGAATCCCACCTTCTCC 50.0 55.0 57.8 58.0 425 bp 1.0 78.6