Hotspot

rrnS-psbI

Genome · 20813-21053 · 241 bp

Back to Gephyrocapsa

Pi 0.0083

MarkerSeek score 57.80

Primer available yes

Label rrnS-psbI

Feature Metadata

label_name: rrnS-psbI
feature_type: igs
region: Genome
length_bp: 241
variable_sites: 3
pi: 0.0083
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrnS-psbI_p1	GCATGTGTTAAGCATACCGC	CCATCTAGAAACCCGAACCG	50.0	55.0	57.9	58.1	335 bp	1.0	79.4
rrnS-psbI_p2	AAACTCTCGTTCGACTTGCA	CCATCTAGAAACCCGAACCG	45.0	55.0	57.8	58.1	352 bp	1.0	78.4
rrnS-psbI_p3	TGTGTTAAGCATACCGCCAG	CCATCTAGAAACCCGAACCG	50.0	55.0	58.3	58.1	332 bp	1.0	78.1
rrnS-psbI_p4	ACTCTCGTTCGACTTGCATG	CCATCTAGAAACCCGAACCG	50.0	55.0	58.3	58.1	350 bp	1.0	77.8
rrnS-psbI_p5	CTCTCGTTCGACTTGCATGT	CCATCTAGAAACCCGAACCG	50.0	55.0	58.3	58.1	349 bp	1.0	77.8