Hotspot

petB-petD

Genome · 5584-5665 · 82 bp

Back to Gephyrocapsa

Pi 0.0163

MarkerSeek score 62.90

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: Genome
length_bp: 82
variable_sites: 2
pi: 0.0163
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	ATTACCCGTTGTAGCAGCAG	TTATGGCCCATACCTTTCGC	50.0	50.0	58.0	58.0	235 bp	1.0	80.3
petB-petD_p2	ATTACCCGTTGTAGCAGCAG	GCACGAAGTTTTGGATCAGC	50.0	50.0	58.0	58.0	208 bp	1.0	80.3
petB-petD_p3	TGACGCGTTTCTATAGTGCC	GCACGAAGTTTTGGATCAGC	50.0	50.0	58.1	58.0	239 bp	1.0	79.8
petB-petD_p4	TGACGCGTTTCTATAGTGCC	TTATGGCCCATACCTTTCGC	50.0	50.0	58.1	58.0	266 bp	1.0	79.7
petB-petD_p5	CGCGTTTCTATAGTGCCCAT	TTATGGCCCATACCTTTCGC	50.0	50.0	58.1	58.0	263 bp	1.0	79.3