Hotspot

psbB-psbT

LSC · 74458-75027 · 570 bp

Back to Geodorum

Pi 0.00585
MarkerSeek score 65.10
Primer available yes
Label psbB-psbT

Feature Metadata

label_name
psbB-psbT
feature_type
igs
region
LSC
length_bp
570
variable_sites
5
pi
0.00585
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB-psbT_p1 TCAGAGATGTTTTTGCCGGT TGGAACTTTAGGTGGTTCTCG 45.0 47.6 57.7 57.6 762-791 bp 1.0 68.8
psbB-psbT_p2 TCAGAGATGTTTTTGCCGGT GGAACTTTAGGTGGTTCTCGA 45.0 47.6 57.7 57.3 761-790 bp 1.0 66.6
psbB-psbT_p3 GCCAGAACCTTGTTCAGAGA TGGAACTTTAGGTGGTTCTCG 50.0 47.6 57.4 57.6 775-804 bp 1.0 66.6
psbB-psbT_p4 GCCAGAACCTTGTTCAGAGA GGAACTTTAGGTGGTTCTCGA 50.0 47.6 57.4 57.3 774-803 bp 1.0 64.4
psbB-psbT_p5 CGCCAGAACCTTGTTCAGAG TGGAACTTTAGGTGGTTCTCG 55.0 47.6 58.8 57.6 776-805 bp 1.0 64.2