Hotspot

psaJ-rpl33

LSC · 67125-67718 · 594 bp

Back to Geodorum

Pi 0.00561

MarkerSeek score 48.70

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 594
variable_sites: 5
pi: 0.00561
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	GCGGCAAAATGAAATCTGCT	AATGCCCGGCGATTCTTTAT	45.0	45.0	58.0	58.0	1054-1059 bp	1.0	80.8
psaJ-rpl33_p2	GCGGCAAAATGAAATCTGCT	ATGCCCGGCGATTCTTTATT	45.0	45.0	58.0	58.0	1053-1058 bp	1.0	80.8
psaJ-rpl33_p3	CGAACTGAACTAAGAGCGCT	AATGCCCGGCGATTCTTTAT	50.0	45.0	58.0	58.0	1420-1425 bp	1.0	80.5
psaJ-rpl33_p4	CGAACTGAACTAAGAGCGCT	ATGCCCGGCGATTCTTTATT	50.0	45.0	58.0	58.0	1419-1424 bp	1.0	80.5
psaJ-rpl33_p5	GCGGCAAAATGAAATCTGCT	GATCGACTCCACTCTCTCCA	45.0	55.0	58.0	58.0	1617-1622 bp	1.0	80.3