Hotspot

ycf4-cemA

LSC · 60771-61526 · 756 bp

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Pi 0.00441

MarkerSeek score 63.20

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 756
variable_sites: 5
pi: 0.00441
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	CATTCCCTTGACCCGTACTG	GGGAGGTAAACCGATGCTTT	55.0	50.0	58.0	57.9	888-891 bp	1.0	80.3
ycf4-cemA_p2	ATTCCCTTGACCCGTACTGA	GGGAGGTAAACCGATGCTTT	50.0	50.0	58.1	57.9	887-890 bp	1.0	80.0
ycf4-cemA_p3	TTCCCTTGACCCGTACTGAT	GGGAGGTAAACCGATGCTTT	50.0	50.0	58.1	57.9	886-889 bp	1.0	80.0
ycf4-cemA_p4	ACAAAAAGCTGCCGAATTGG	GGGAGGTAAACCGATGCTTT	45.0	50.0	57.8	57.9	840-843 bp	1.0	79.3
ycf4-cemA_p5	TATTTCTTGCGCGTACCCAT	GGGAGGTAAACCGATGCTTT	45.0	50.0	57.7	57.9	818-821 bp	1.0	78.0