Hotspot

rbcL-accD

LSC · 56945-57782 · 838 bp

Back to Geodorum

Pi 0.00796

MarkerSeek score 51.30

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 838
variable_sites: 10
pi: 0.00796
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	GCCGCTGCTTGTGAAATATG	CCACTGGTATGTCCAAGAGC	50.0	55.0	58.2	58.0	1023-1032 bp	1.0	79.2
rbcL-accD_p2	GCCGCTGCTTGTGAAATATG	AGTTTCTTCGCTTCCACTGG	50.0	50.0	58.2	58.1	1036-1045 bp	1.0	78.5
rbcL-accD_p3	GCTAGCAAATGGAGCTCTGA	CCACTGGTATGTCCAAGAGC	50.0	55.0	57.7	58.0	1047-1056 bp	1.0	77.9
rbcL-accD_p4	TAGCAAATGGAGCTCTGAGC	CCACTGGTATGTCCAAGAGC	50.0	55.0	57.7	58.0	1045-1054 bp	1.0	77.9
rbcL-accD_p5	AGCTAGCAAATGGAGCTCTG	CCACTGGTATGTCCAAGAGC	50.0	55.0	57.7	58.0	1048-1057 bp	1.0	77.9