Hotspot

trnR-UCU-atpA

LSC · 9658-9799 · 142 bp

Back to Geodorum

Pi 0.00939
MarkerSeek score 59.30
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
142
variable_sites
2
pi
0.00939
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 ATGAAGAGCGTCCATTGTCT CCGAAGAAGCTGAAACCCTT 45.0 50.0 56.9 58.1 298 bp 1.0 71.4
trnR-UCU-atpA_p2 ATGAAGAGCGTCCATTGTCT TCACCGAAGAAGCTGAAACC 45.0 50.0 56.9 58.1 301 bp 1.0 71.3
trnR-UCU-atpA_p3 AGGAATGAAGAGCGTCCATT CCGAAGAAGCTGAAACCCTT 45.0 50.0 56.9 58.1 302 bp 1.0 71.3
trnR-UCU-atpA_p4 AGGAATGAAGAGCGTCCATT TCACCGAAGAAGCTGAAACC 45.0 50.0 56.9 58.1 305 bp 1.0 71.1
trnR-UCU-atpA_p5 GGAATGAAGAGCGTCCATTG CCGAAGAAGCTGAAACCCTT 50.0 50.0 56.9 58.1 301 bp 1.0 71.0