Hotspot

trnS-GCU-trnG-GCC

LSC · 8116-8681 · 566 bp

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Pi 0.013

MarkerSeek score 60.50

Primer available yes

Label trnS-GCU-trnG-GCC

Feature Metadata

label_name: trnS-GCU-trnG-GCC
feature_type: igs
region: LSC
length_bp: 566
variable_sites: 11
pi: 0.013
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-GCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	635 bp	1.0	53.1
trnS-GCU-trnG-GCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	634 bp	1.0	53.1
trnS-GCU-trnG-GCC_p3	GGGATTTGAACCCTCGGTAC	ACGAATCACACTTTTACCACT	55.0	38.1	58.0	55.5	678 bp	1.0	52.7
trnS-GCU-trnG-GCC_p4	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	637 bp	1.0	52.0
trnS-GCU-trnG-GCC_p5	TCTCAAGTGATCAGAGGGGG	ACGAATCACACTTTTACCACT	55.0	38.1	58.1	55.5	709 bp	1.0	51.9