Hotspot

ycf3

LSC · 41518-43550 · 2033 bp

Back to Gentianopsis

Pi 0.000328

MarkerSeek score 32.60

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 2033
variable_sites: 1
pi: 0.000328
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 32.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	AGCGTCCTTCCTCTTTTATT	TCTCACGAGGGGTAATGAGA	40.0	50.0	54.4	57.2	2106-2107 bp	1.0	44.3
ycf3_p2	AGCGTCCTTCCTCTTTTATTT	TCTCACGAGGGGTAATGAGA	38.1	50.0	55.2	57.2	2106-2107 bp	1.0	43.2
ycf3_p3	AGCGTCCTTCCTCTTTTATT	TCACGAGGGGTAATGAGAAGA	40.0	47.6	54.4	57.9	2104-2105 bp	1.0	42.1
ycf3_p4	AGCGTCCTTCCTCTTTTATT	TCTCACGAGGGGTAATGAGAA	40.0	47.6	54.4	57.9	2106-2107 bp	1.0	42.1
ycf3_p5	AGCGTCCTTCCTCTTTTATT	TTCTCACGAGGGGTAATGAGA	40.0	47.6	54.4	57.9	2107-2108 bp	1.0	42.1