Hotspot

ycf1

SSC · 121412-126889 · 5478 bp

Back to Gentianopsis

Pi 0.00427

MarkerSeek score 55.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5478
variable_sites: 35
pi: 0.00427
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCCCCCTTTACTTTTGATCCTC	TGGCTCTTCCCAGAATCTTTG	45.5	47.6	57.4	57.9	320 bp	1.0	51.9
ycf1_p2	CCCCCTTTACTTTTGATCCTCT	TGGCTCTTCCCAGAATCTTTG	45.5	47.6	57.4	57.9	319 bp	1.0	51.9
ycf1_p3	TCCCCCTTTACTTTTGATCCTC	GATCTTTGGCTCTTCCCAGA	45.5	50.0	57.4	56.9	326 bp	1.0	51.8
ycf1_p4	CCCCCTTTACTTTTGATCCTCT	GATCTTTGGCTCTTCCCAGA	45.5	50.0	57.4	56.9	325 bp	1.0	51.8
ycf1_p5	TCCCCCTTTACTTTTGATCCTC	AGATCTTTGGCTCTTCCCAG	45.5	50.0	57.4	56.9	327 bp	1.0	51.8