Hotspot

psaJ-rpl33

LSC · 66161-66565 · 405 bp

Back to Gentianopsis

Pi 0.00494
MarkerSeek score 44.00
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
405
variable_sites
3
pi
0.00494
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TTCCTTGTTACTGCGCCTTT GAAATGCCCGTTGATACCCT 45.0 50.0 58.0 57.9 1148-1153 bp 1.0 79.9
psaJ-rpl33_p2 TTGTTACTGCGCCTTTGAGT GAAATGCCCGTTGATACCCT 45.0 50.0 58.0 57.9 1144-1149 bp 1.0 79.8
psaJ-rpl33_p3 TTCCTTGTTACTGCGCCTTT CGGTTAACTCGGCTCTTTCA 45.0 50.0 58.0 57.9 1740-1743 bp 1.0 79.8
psaJ-rpl33_p4 TGCATTGTTCCACAGTGTCA GAAATGCCCGTTGATACCCT 45.0 50.0 58.0 57.9 1034-1039 bp 1.0 79.7
psaJ-rpl33_p5 TTGTTACTGCGCCTTTGAGT CGGTTAACTCGGCTCTTTCA 45.0 50.0 58.0 57.9 1736-1739 bp 1.0 79.7