Hotspot

accD-psaI

LSC · 57792-58395 · 604 bp

Back to Gentianopsis

Pi 0.00111

MarkerSeek score 59.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 604
variable_sites: 1
pi: 0.00111
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	TTTGACCTAATCGTACCGCG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.1	58.0	772-773 bp	1.0	79.3
accD-psaI_p2	TTTGACCTAATCGTACCGCG	AGAAACCATTGCAATTGCCG	50.0	45.0	58.1	57.9	777-778 bp	1.0	78.8
accD-psaI_p3	TGACCTAATCGTACCGCGTA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.3	58.0	770-771 bp	1.0	77.3
accD-psaI_p4	TGACCTAATCGTACCGCGTA	AGAAACCATTGCAATTGCCG	50.0	45.0	58.3	57.9	775-776 bp	1.0	76.8
accD-psaI_p5	TTTGACCTAATCGTACCGCG	GCCGGAAATAGTAGGCCTAC	50.0	55.0	58.1	57.5	761-762 bp	1.0	75.9