Hotspot

trnS-UGA-psbZ

LSC · 34532-34865 · 334 bp

Back to Gentianopsis

Pi 0.00399

MarkerSeek score 55.80

Primer available yes

Label trnS-UGA-psbZ

Feature Metadata

label_name: trnS-UGA-psbZ
feature_type: igs
region: LSC
length_bp: 334
variable_sites: 2
pi: 0.00399
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-psbZ_p1	CAACCACTCAGCCATCTCTC	ACAACAGGCACGCTAATCAA	55.0	45.0	58.0	58.1	432 bp	1.0	79.3
trnS-UGA-psbZ_p2	CAACCACTCAGCCATCTCTC	CCAACCATCAGGAGAAGCAA	55.0	50.0	58.0	57.8	454 bp	1.0	78.5
trnS-UGA-psbZ_p3	CAACCACTCAGCCATCTCTC	AACCAACCATCAGGAGAAGC	55.0	50.0	58.0	57.8	456 bp	1.0	78.5
trnS-UGA-psbZ_p4	GTTTTCAAGACCGGGGCTAT	ACAACAGGCACGCTAATCAA	50.0	45.0	57.9	58.1	452 bp	1.0	78.5
trnS-UGA-psbZ_p5	GTTTTCAAGACCGGGGCTAT	CCAACCATCAGGAGAAGCAA	50.0	50.0	57.9	57.8	474 bp	1.0	77.7