Hotspot

trnE-UUC-trnT-GGU

LSC · 29593-30386 · 794 bp

Back to Gentianopsis

Pi 0.00383

MarkerSeek score 55.90

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 794
variable_sites: 4
pi: 0.00383
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GCTGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.3	58.0	870-897 bp	1.0	80.6
trnE-UUC-trnT-GGU_p2	TGTCCTGAACCACTAGACGA	GAACCGATGACTTACGCCTT	50.0	50.0	57.7	58.0	849-876 bp	1.0	80.6
trnE-UUC-trnT-GGU_p3	GCTGCCTCCTTGAAAGAGAG	CCGATGACTTACGCCTTACC	55.0	55.0	58.3	58.2	867-894 bp	1.0	79.5
trnE-UUC-trnT-GGU_p4	GCTGCCTCCTTGAAAGAGAG	AAACCAAAGCCCCTTATCGG	55.0	50.0	58.3	58.2	894-921 bp	1.0	79.4
trnE-UUC-trnT-GGU_p5	TGTCCTGAACCACTAGACGA	CCGATGACTTACGCCTTACC	50.0	55.0	57.7	58.2	846-873 bp	1.0	79.4