Hotspot

psbM-trnD-GUC

LSC · 28323-29177 · 855 bp

Back to Gentianopsis

Pi 0.0039

MarkerSeek score 47.30

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 855
variable_sites: 5
pi: 0.0039
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AATGCCCGCAAAACTACTCA	CACCTATTCAATTCCCGGGG	45.0	55.0	58.1	58.0	1559-1574 bp	1.0	79.5
psbM-trnD-GUC_p2	ATGCCCGCAAAACTACTCAA	CACCTATTCAATTCCCGGGG	45.0	55.0	58.1	58.0	1558-1573 bp	1.0	79.5
psbM-trnD-GUC_p3	TGCCCGCAAAACTACTCAAT	CACCTATTCAATTCCCGGGG	45.0	55.0	58.1	58.0	1557-1572 bp	1.0	79.5
psbM-trnD-GUC_p4	AAAAATCGGCGAGGCTTACT	CACCTATTCAATTCCCGGGG	45.0	55.0	57.9	58.0	1606-1621 bp	1.0	79.4
psbM-trnD-GUC_p5	TGCCCGCAAAACTACTCAAT	ACCTATTCAATTCCCGGGGA	45.0	50.0	58.1	58.1	1556-1571 bp	1.0	78.9