Hotspot

trnH-GUG-psbA

LSC · 75-483 · 409 bp

Back to Gentianopsis

Pi 0.00581

MarkerSeek score 59.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 409
variable_sites: 3
pi: 0.00581
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	TTCCTCTAGACCTAGCTGCT	50.0	50.0	57.9	56.9	436-485 bp	1.0	74.4
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	TTCCTCTAGACCTAGCTGCT	50.0	50.0	58.2	56.9	437-486 bp	1.0	74.2
trnH-GUG-psbA_p3	CTGCCTTAATCCACTTGGCT	ACCGTGCTAATCTTGGTATGG	50.0	47.6	57.9	57.8	487-536 bp	1.0	73.8
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	ACCGTGCTAATCTTGGTATGG	50.0	47.6	58.2	57.8	488-537 bp	1.0	73.6
trnH-GUG-psbA_p5	AATCCACTTGGCTACATCCG	TTCCTCTAGACCTAGCTGCT	50.0	50.0	57.7	56.9	429-478 bp	1.0	72.8