Hotspot

ycf1

SSC · 120278-125761 · 5484 bp

Back to Gentiana

Pi 0.0429

MarkerSeek score 72.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5484
variable_sites: 1302
pi: 0.0429
unique_haplotype_count: 41
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	CATCGCCCCATCTCAATTGA	TTGGACGGGAATGAATGAGG	50.0	50.0	58.0	57.6	308-443 bp	0.906977	84.7
ycf1_p2	CATCGCCCCATCTCAATTGA	TGGACGGGAATGAATGAGGA	50.0	50.0	58.0	58.4	307-442 bp	0.906977	84.7
ycf1_p3	CATCGCCCCATCTCAATTGA	AAGTGTCCCATCTCAACCCT	50.0	50.0	58.0	58.3	280-415 bp	0.581395	73.0
ycf1_p4	GTTGATTCAAAGCATCGCCC	AAGTGTCCCATCTCAACCCT	50.0	50.0	58.1	58.3	421-427 bp	0.209302	53.7
ycf1_p5	TGACTACAGTTATTCGCGGC	AAGTGTCCCATCTCAACCCT	50.0	50.0	58.1	58.3	848 bp	0.023256	38.2