Hotspot

psaI-ycf4

LSC · 57986-58402 · 417 bp

Back to Gentiana

Pi 0.051
MarkerSeek score 72.60
Primer available yes
Label psaI-ycf4

Feature Metadata

label_name
psaI-ycf4
feature_type
igs
region
LSC
length_bp
417
variable_sites
102
pi
0.051
unique_haplotype_count
25
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaI-ycf4_p1 ATTTCCGGCAATTGCAATGG AGACCCCGCGATAAGTTCTA 45.0 50.0 58.0 57.9 462-544 bp 0.976744 90.3
psaI-ycf4_p2 ATTTCCGGCAATTGCAATGG TAGACCCCGCGATAAGTTCT 45.0 50.0 58.0 57.9 463-545 bp 0.976744 90.3
psaI-ycf4_p3 ATTTCCGGCAATTGCAATGG TCTAGACCCCGCGATAAGTT 45.0 50.0 58.0 57.9 465-547 bp 0.976744 90.3
psaI-ycf4_p4 ATTTCCGGCAATTGCAATGG TTCTAGACCCCGCGATAAGT 45.0 50.0 58.0 57.9 466-548 bp 0.976744 90.3
psaI-ycf4_p5 GGCAATTGCAATGGCTTCTT AGACCCCGCGATAAGTTCTA 45.0 50.0 57.9 57.9 456-538 bp 0.976744 89.7