Hotspot

trnL-UAA-trnF-GAA

LSC · 46617-46957 · 341 bp

Back to Gentiana

Pi 0.0555

MarkerSeek score 71.80

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 341
variable_sites: 108
pi: 0.0555
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	TCAAGTCCCTCTATCCCCAC	TCTACCAGCTGAGCTATCCC	55.0	55.0	57.8	58.0	385 bp	0.023256	39.5
trnL-UAA-trnF-GAA_p2	TCAAGTCCCTCTATCCCCAC	TTTGAACTGGTGACACGAGG	55.0	50.0	57.8	58.1	421 bp	0.023256	39.1
trnL-UAA-trnF-GAA_p3	TCAAGTCCCTCTATCCCCAC	GTGCCAGGAACCAGATTTGA	55.0	50.0	57.8	58.1	436 bp	0.023256	38.8
trnL-UAA-trnF-GAA_p4	TCAAGTCCCTCTATCCCCAC	TCAGTCCTCTGCTCTACCAG	55.0	55.0	57.8	57.9	397 bp	0.023256	38.4
trnL-UAA-trnF-GAA_p5	TCAAGTCCCTCTATCCCCAC	CATGTGCCAGGAACCAGATT	55.0	50.0	57.8	58.2	439 bp	0.023256	38.3