Hotspot

psaA-ycf3

LSC · 40594-41443 · 850 bp

Back to Gentiana

Pi 0.0443

MarkerSeek score 72.40

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 850
variable_sites: 204
pi: 0.0443
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	TGTGAGTTGGATCGCTTAGC	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.0	58.0	1180-1279 bp	0.604651	73.4
psaA-ycf3_p2	ATGTATTTGATGTCCCGCCC	ATCAAGCCGCTGAGTATTGG	50.0	50.0	58.0	58.0	1506-1605 bp	0.604651	72.4
psaA-ycf3_p3	CTTCTGGTTCCGGCGAAC	ACGAGGCGTTTCGAATAA	61.1	44.4	58.4	54.4	789-915 bp	1.0	52.0
psaA-ycf3_p4	ACTTCTGGTTCCGGCGAA	ACGAGGCGTTTCGAATAA	55.6	44.4	58.9	54.4	790-916 bp	1.0	52.0
psaA-ycf3_p5	ACTTCTGGTTCCGGCGAAC	ACGAGGCGTTTCGAATAA	57.9	44.4	60.3	54.4	790-916 bp	1.0	52.0