Hotspot

trnT-GGU-psbD

LSC · 30332-31542 · 1211 bp

Back to Gentiana

Pi 0.0414

MarkerSeek score 76.00

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1211
variable_sites: 289
pi: 0.0414
unique_haplotype_count: 37
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 76.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GGCGTAAGTCATCGGTTCAA	TTTACCAAGGGCTATAGTCA	50.0	40.0	58.3	53.0	903-1278 bp	1.0	52.0
trnT-GGU-psbD_p2	GTGGTAGAGTAACGCCATGG	TTTACCAAGGGCTATAGTCA	55.0	40.0	58.1	53.0	926-1301 bp	0.976744	51.1
trnT-GGU-psbD_p3	TCAGTGGTAGAGTAACGCCA	TTTACCAAGGGCTATAGTCA	50.0	40.0	58.1	53.0	929-1304 bp	0.976744	51.1
trnT-GGU-psbD_p4	GTGGTAGAGTAACGCCATGG	TTACCAAGGGCTATAGTCAT	55.0	40.0	58.1	52.8	925-1300 bp	0.976744	51.1
trnT-GGU-psbD_p5	TCAGTGGTAGAGTAACGCCA	TTACCAAGGGCTATAGTCAT	50.0	40.0	58.1	52.8	928-1303 bp	0.976744	51.1