Hotspot

rpoB-trnC-GCA

LSC · 25324-26569 · 1246 bp

Back to Gentiana

Pi 0.0447

MarkerSeek score 78.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1246
variable_sites: 286
pi: 0.0447
unique_haplotype_count: 35
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CCTCATTTCCATCAACGAGC	CCGGATTTGAACTGGGGAAA	50.0	50.0	56.9	58.1	874-1360 bp	0.930233	79.6
rpoB-trnC-GCA_p2	ACTCCCTCATTTCCATCAACG	CCGGATTTGAACTGGGGAAA	47.6	50.0	58.0	58.1	878-1364 bp	0.883721	78.6
rpoB-trnC-GCA_p3	TCCCTCATTTCCATCAACGAG	CCGGATTTGAACTGGGGAAA	47.6	50.0	57.7	58.1	876-1362 bp	0.930233	78.4
rpoB-trnC-GCA_p4	ACTCCCTCATTTCCATCAACG	AAAAGGATTTGCAGTCCCCC	47.6	50.0	58.0	58.4	860-1346 bp	0.906977	77.4
rpoB-trnC-GCA_p5	CTCCCTCATTTCCATCAACGA	CCGGATTTGAACTGGGGAAA	47.6	50.0	57.7	58.1	877-1363 bp	0.883721	76.6