Hotspot

trnK-UUU-trnQ-UUG

LSC · 4278-5619 · 1342 bp

Back to Gentiana

Pi 0.0467

MarkerSeek score 74.10

Primer available yes

Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name: trnK-UUU-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1342
variable_sites: 315
pi: 0.0467
unique_haplotype_count: 38
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-trnQ-UUG_p1	CCGTTGAGTTAGCAACCC	GAGGTTCGAATCCTTCCGTC	55.6	55.0	55.7	58.1	478-1495 bp	1.0	57.0
trnK-UUU-trnQ-UUG_p2	CCGTTGAGTTAGCAACCC	TTCGGAGGTTCGAATCCTTC	55.6	50.0	55.7	57.3	482-1499 bp	1.0	52.2
trnK-UUU-trnQ-UUG_p3	CCGTTGAGTTAGCAACCC	TAAGGCAACGGGTTTTGGTC	55.6	50.0	55.7	58.7	508-1525 bp	1.0	52.1
trnK-UUU-trnQ-UUG_p4	CCGTTGAGTTAGCAACCC	GGGTTTTGGTCCCGCTATTC	55.6	55.0	55.7	58.9	499-1516 bp	1.0	52.0
trnK-UUU-trnQ-UUG_p5	CCGTTGAGTTAGCAACCC	AAATCTAAATGGGGCGTGGC	55.6	50.0	55.7	58.9	535-1552 bp	0.976744	51.1