Hotspot

trnH-GUG-psbA

LSC · 76-439 · 364 bp

Back to Gentiana

Pi 0.0963

MarkerSeek score 68.50

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 364
variable_sites: 138
pi: 0.0963
unique_haplotype_count: 36
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	TTCGGCGGTTCCTTATTCAG	50.0	50.0	57.9	58.0	657-875 bp	1.0	90.8
trnH-GUG-psbA_p2	CTGCCTTAATCCACTTGGCT	TCAGTGCTATGCATGGTTCC	50.0	50.0	57.9	58.0	641-859 bp	1.0	90.6
trnH-GUG-psbA_p3	ACTGCCTTAATCCACTTGGC	TTCGGCGGTTCCTTATTCAG	50.0	50.0	58.2	58.0	658-876 bp	1.0	90.6
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	CAGTGCTATGCATGGTTCCT	50.0	50.0	57.9	58.0	640-858 bp	1.0	90.5
trnH-GUG-psbA_p5	CTGCCTTAATCCACTTGGCT	TGCTGCTTGGCCTGTAATAG	50.0	50.0	57.9	58.0	448-666 bp	0.651163	76.6