Hotspot

ndhK

LSC · 47459-47883 · 425 bp

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Pi 0.166

MarkerSeek score 71.10

Primer available yes

Label ndhK

Feature Metadata

label_name: ndhK
feature_type: gene
region: LSC
length_bp: 425
variable_sites: 204
pi: 0.166
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhK_p1	AGTTGGGCGGAAATGACTTT	CGTAGAGAACCAACCCTTCG	45.0	55.0	58.0	58.0	1405-1474 bp	0.714286	76.5
ndhK_p2	CCTCGTGTCACCAGTTCAAA	CGTAGAGAACCAACCCTTCG	50.0	55.0	58.1	58.0	1111-1185 bp	0.714286	76.1
ndhK_p3	AGTTGGGCGGAAATGACTTT	TGCATGGGGAAAGAAAGCAT	45.0	45.0	58.0	58.1	1015-1035 bp	0.571429	68.0
ndhK_p4	GTTTCAGTAGGTGGGCCAAA	CGTAGAGAACCAACCCTTCG	50.0	55.0	58.0	58.0	987-992 bp	0.285714	52.2
ndhK_p5	TTCAGTAGGTGGGCCAAAAC	CGTAGAGAACCAACCCTTCG	50.0	55.0	58.0	58.0	985-990 bp	0.285714	52.2