Hotspot

rps15-ycf1

SSC · 109844-110225 · 382 bp

Back to Genlisea

Pi 0.136

MarkerSeek score 77.20

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 382
variable_sites: 134
pi: 0.136
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAGCAGTCGTTGTCGTTTTC	TGCAACGGAAAGAGTGTCAA	50.0	45.0	58.0	58.0	789-790 bp	0.285714	52.7
rps15-ycf1_p2	CAGCAGTCGTTGTCGTTTTC	TTGCAACGGAAAGAGTGTCA	50.0	45.0	58.0	58.0	790-791 bp	0.285714	52.7
rps15-ycf1_p3	AGTCGTTGTCGTTTTCCCAA	TGCAACGGAAAGAGTGTCAA	45.0	45.0	58.0	58.0	785-786 bp	0.285714	52.5
rps15-ycf1_p4	AGTCGTTGTCGTTTTCCCAA	TTGCAACGGAAAGAGTGTCA	45.0	45.0	58.0	58.0	786-787 bp	0.285714	52.5
rps15-ycf1_p5	TAGTTCAGCAGTCGTTGTCG	TGCAACGGAAAGAGTGTCAA	50.0	45.0	58.0	58.0	794-795 bp	0.285714	52.3