Hotspot

rpl32-trnL-UAG

SSC · 105941-106456 · 516 bp

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Pi 0.162
MarkerSeek score 81.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
516
variable_sites
209
pi
0.162
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 ACAACTCTTTGGCAGCGTTA GGATTCGAACCGAGATGCTT 45.0 50.0 58.0 58.1 632-711 bp 0.571429 74.1
rpl32-trnL-UAG_p2 AATCCAGTCGGTCATTGTGG TTCGAACCGAGATGCTTGAG 50.0 50.0 57.9 58.0 1217-1394 bp 0.571429 73.9
rpl32-trnL-UAG_p3 ACAACTCTTTGGCAGCGTTA TTCGAACCGAGATGCTTGAG 45.0 50.0 58.0 58.0 629-708 bp 0.428571 68.2
rpl32-trnL-UAG_p4 TCGCTTTCTACGGGTAATGC TTCGAACCGAGATGCTTGAG 50.0 50.0 58.1 58.0 642-667 bp 0.285714 54.5
rpl32-trnL-UAG_p5 TCGCTTTCTACGGGTAATGC GGATTCGAACCGAGATGCTT 50.0 50.0 58.1 58.1 645-670 bp 0.285714 54.1